tβri ca mice  (Inserm Transfert)

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Nucleotide sequences of qPCR primers.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques:

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Expression analysis of TβRI CA transgenes in livers of TβRI CA /Fsp1-Cre mice. (A) Detection of TβRI CA transcripts by qPCR performed on total RNA from livers of wild-type and TβRI CA /Fsp1-Cre mice. (B) Expression of Tgf-β, Snail1, Twist and PAI-1 in livers of wild-type and TβRI CA /Fsp1-Cre mice (n = 6). (C) Phosphorylated form of Smad2 and 3 (pSmad2/3) level was analyzed by Western blot analysis. β-actin proteins served as a control. Non-adjusted images of pSmad2/3 and β-actin Western blots can be found in supplemental materials. (D) Hematoxylin/Eosin and Sirius Red/Fast green staining of liver sections obtained from control and TβRI CA /Fsp1-Cre mice. The mRNA expression of TβRI CA (E) and Snail1 (F) in macrophages isolated from livers of wild-type and TβRI CA /Fsp1-Cre mice (n = 6). Data are shown as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01, ∗∗∗∗ P < 0.0001 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Expressing, Western Blot, Control, Staining, Isolation

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Fsp1-Cre- mediated expression of TβRI CA is protective against conA-induced liver injury. (A–B) Serum level of aspartate transaminase (AST) and alanine transaminase (ALT) in control and TβRI CA /Fsp1-Cre mice treated with PBS or conA for 6 h. AST (A) and ALT (B) level was lower in TβRI CA /Fsp1-Cre mice compared to those of control mice. Data are means ± SD of n = 10 per experimental group. (C) Survival experiments were performed in wild-type and TβRI CA /Fsp1-Cre mice treated with conA 20 mg/kg body weight (n = 11–12 per group). ∗∗∗ P < 0.001, ∗∗∗∗ P < 0.0001 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Expressing, Control

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Assessment of liver histopathology following conA treatment. (A) Paraffin sections of liver from conA-treated wild-type and TβRI CA /Fsp1-Cre mice. The fraction of necrotic area was enclosed by dash lines and percentage necrotic area was calculated by examining 10 high-power fields/livers (B). Data are shown as means ± SD. ∗∗∗∗ P < 0.0001 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Histopathology

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Hepatic immune infiltration induced by conA. Hematoxylin and Eosin-stained liver sections obtained from conA-treated wild-type (A) and TβRI CA /Fsp1-Cre mice (B). Myeloperoxidase immunohistochemical staining of liver sections from conA-treated wild-type (C) and TβRI CA /Fsp1-Cre mice (D). CD3 immunohistochemical staining of liver sections from conA-treated wild-type (E) and TβRI CA /Fsp1-Cre mice (F). Quantification of myeloperoxidase-(G), and CD3-(F) positive cells in livers from PBS-treated and conA-treated mice (n = 6). Flow cytometry analysis of CD4 + cells (I), CD4 + IFN-γ + cells (J) and CD4 + IL-4 + cells (K) in livers from conA-treated wild-type and TβRI CA /Fsp1-Cre mice (n = 6). (L) Representative results of TCR-β and NK1.1 positivity by flow cytometry. (M) Quantitative analysis of TCR-β- and NK1.1-positive NKT cells by flow cytometry (n = 6). Data are shown as means ± SD. ∗ P < 0.05, ∗∗∗∗ P < 0.0001 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Staining, Immunohistochemical staining, Flow Cytometry

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: M2 macrophage polarization was enhanced in livers of conA-treated TβRI CA /Fsp1-Cre mice. (A) Representative results of F4/80 and CD163 positivity in liver macrophages by flow cytometry. (B) Quantitative analysis of F4/80- and CD163-positive liver macrophages by flow cytometry (n = 6). Expression of CD206 (C) and CCR2 (D) in livers of PBS- and conA-treated wild-type and TβRI CA /Fsp1-Cre mice (n = 6). Data are shown as means ± SD. ∗ P < 0.05, ∗∗ P < 0.01 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Flow Cytometry, Expressing

Journal: Heliyon

Article Title: Macrophage expression of constitutively active TβRI alleviates hepatic injury in a mouse model of concanavalin A-induced autoimmune hepatitis

doi: 10.1016/j.heliyon.2025.e42691

Figure Lengend Snippet: Gene expression analysis of liver macrophages from conA-treated TβRI CA /Fsp1-Cre mice. (A) Detection of Arg 1, Ym1 , and CD206 transcripts by qPCR performed on total RNA from liver macrophages of conA-treated mice. (B) Expression of H2-Aa and H2-k1 in liver macrophages from conA-treated wild-type and TβRI CA /Fsp1-Cre mice. (C) Expression of FOXO1 and IRF1 in liver macrophages from conA-treated wild-type and TβRI CA /Fsp1-Cre mice. (D) Quantification of CD1d mRNA expression levels in liver macrophages from conA-treated wild-type and TβRI CA /Fsp1-Cre mice. Data are shown as means ± SD of n = 5 per experimental group. ∗ P < 0.05, ∗∗ P < 0.001, ∗∗∗ P < 0.001 between the two indicated groups.

Article Snippet: TβRI CA mice were kindly provided by Professor Laurent Bartholin, INSERM, France.

Techniques: Gene Expression, Expressing